Live-cell imaging of conidial anastomosis tube fusion during colony initiation in Fusarium oxysporum.

TitleLive-cell imaging of conidial anastomosis tube fusion during colony initiation in Fusarium oxysporum.
Publication TypeJournal Article
Year of Publication2018
AuthorsKurian, SM, Di Pietro, A, Read, ND
JournalPLoS One
Volume13
Issue5
Paginatione0195634
Date Published2018
ISSN1932-6203
Abstract

Fusarium oxysporum exhibits conidial anastomosis tube (CAT) fusion during colony initiation to form networks of conidial germlings. Here we determined the optimal culture conditions for this fungus to undergo CAT fusion between microconidia in liquid medium. Extensive high resolution, confocal live-cell imaging was performed to characterise the different stages of CAT fusion, using genetically encoded fluorescent labelling and vital fluorescent organelle stains. CAT homing and fusion were found to be dependent on adhesion to the surface, in contrast to germ tube development which occurs in the absence of adhesion. Staining with fluorescently labelled concanavalin A indicated that the cell wall composition of CATs differs from that of microconidia and germ tubes. The movement of nuclei, mitochondria, vacuoles and lipid droplets through fused germlings was observed by live-cell imaging.

DOI10.1371/journal.pone.0195634
Alternate JournalPLoS ONE
PubMed ID29734342
PubMed Central IDPMC5937734