|Title||Electrophilic, Activation-Free Fluorogenic Reagent for Labeling Bioactive Amines.|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Sintes, M, De Moliner, F, Caballero-Lima, D, Denning, DW, Read, ND, Kielland, N, Vendrell, M, Lavilla, R|
|Date Published||2016 Jun 15|
Herein we report the preparation of BODIPY mesoionic acid fluorides through a short sequence involving an isocyanide multicomponent reaction as the key synthetic step. These novel BODIPY acid fluorides are water-stable electrophilic reagents that can be used for the fluorescent derivatization of amine-containing biomolecules using mild and activation-free reaction conditions. As a proof of principle, we have labeled the antifungal natamycin and generated a novel fluorogenic probe for imaging a variety of human and plant fungal pathogens, with excellent selectivity over bacterial cells.
|Alternate Journal||Bioconjug. Chem.|